Emeritus Laboratory Rolf Kemler
The laboratory is interested in cell adhesion molecules as morphoregulators, specifically in a structural and functional analysis of how the cadherin-catenin-cell adhesion complex regulates cell adhesion and signaling during embryonic development. Particular emphasis is put on the dual function of beta-catenin, which is of crucial importance in the cadherin adhesion complex and plays a central role in the Wnt-signaling pathway. Techniques used include homologous recombination in embryonic stem (ES) cells, heterotypic expression, biochemical and immunochemical investigations of protein interactions of the cadherin-catenin-cell adhesion complex with cytoskeletal actin microfilaments, and the activation of target (or reporter) gene expression by nuclear beta-catenin. The Cre/loxP system is used to conditionally inactivate E-cadherin and beta-catenin in specific cell lineages and tissues.
Senior Group Leader
born in Burghaun, Germany
studies in veterinary medicine, University Giessen, Germany
PhD at the Max Planck Institute of Immunobiology, Freiburg, Germany
Postdoctoral studies at the Institut Pasteur, Paris, France
Group Leader, Friedrich-Miescher-Laboratorium of the Max Planck Society in Tübingen
1987 - 1992
Group Leader at the Max Planck Institute of Immunobiology, Freiburg, Germany
Director at the Max Planck Institute of Immunobiology and Epigenetics, Freiburg, Germany
Cell Adhesion in Mouse Development (Marc Stemmler)
Classical cadherins and their molecular function during development and differentiation – Marc Stemmler. Cadherin-mediated adhesion is crucial during...
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Post-transcriptional control of ß-catenin and N-cadherin by microRNAs and RNA-binding proteins (Jennifer Winter)
The regulation of gene expression at the post-transcriptional level is critical to normal development and tissue homeostasis. We are examining a putative...
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The function of ß-catenin in embryonic stem (ES) cells and in development (Rolf Kemler)
The dual role of ß-catenin in cadherin-mediated adhesion and as the downstream effector of the canonical Wnt signaling pathway is addressed by combining...
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Wnt/ß-catenin signaling regulates telomerase in stem cells and cancer cells.
Hoffmeyer, K., Raggioli, A., Rudloff, S., Anton, R., Hierholzer, A., Del Valle, I., Hein, K., Vogt, R., Kemler, R. (2012)
Science 336, 1549-1554
Lineage specification of parietal epithelial cells requires ß-catenin/Wnt signaling
Grouls S, Iglesias DM, Wentzensen N, Moeller MJ, Bouchard M, Kemler R, Goodyer P, Niggli F, Gröne HJ, Kriz W, Koesters R (2012)
J Am Soc Nephrol. 2012 Jan;23(1):63-72
Differential requirements for ß-catenin during mouse development
Rudloff S, Kemler R (2012)
Development. 2012 Oct;139(20):3711-21
N-cadherin can structurally substitute for E-cadherin during intestinal development but leads to polyp formation.
Libusova, L., Stemmler, M.P., Hierholzer, A., Schwarz, H. and Kemler, R. (2010)
Development 137, 2297-2305
Nanog is required for primitive endoderm formation through a non-cell autonomous mechanism.
Messerschmidt, D.M. and Kemler, R. (2010)
Dev Biol. 344, 129-137
Beta-catenin-mediated signaling and cell adhesion in postgastrulation mouse embryos.
Hierholzer, A. and Kemler, R. (2010)
Dev. Dyn. 239, 191-199
Abrogation of E-cadherin-mediated cell-cell contact in mouse embryonic stem cells results in reversible LIF-independent self-renewal.
Soncin, F., Mohamet, L., Eckardt, D., Ritson, S., Eastham, A.M., Bobola, N., Russell, A., Davies, S., Kemler, R., Merry, C.L. and Ward, C.M. (2010)
Stem Cells 27, 2069-2080
E-cadherin as a novel surface marker of spermatogonial stem cells.
Tolkunova, E.N., Malashicheva, A.B., Chikhirzhina, E.V., Kostyleva, E.I., Zeng, W., Luo, J., Dobrinski?, I., Hierholzer, A., Kemler, R. and Tomilin, A.N. (2009)
Tsitologiia 51, 212-218
Cdx1::Cre allele for gene analysis in the extraembryonic ectoderm and the three germ layers of mice at mid-gastrulation.
Hierholzer, A. and Kemler, R. (2009)
Genesis 47, 204-209
Neural progenitors of the postnatal and adult mouse forebrain retain the ability to self-replicate, form neurospheres, and undergo multipotent differentiation in vivo.
Neumeister, B., Grabosch, A., Basak, O., Kemler, R. and Taylor, V. (2009)
Stem Cells 27, 714-723
The epithelial cell adhesion molecule EpCAM is required for epithelial morphogenesis and integrity during zebrafish epiboly and skin development.
Slanchev, K., Carney, T. J., Stemmler, M. P., Koschorz, B., Amsterdam, A., Schwarz, H. and Hammerschmidt, M. (2009)
PLoS Genet 5, e1000563
The EMT-activator ZEB1 promotes tumorigenicity by repressing stemness-inhibiting microRNAs.
Wellner, U., Schubert, J., Burk, U. C., Schmalhofer, O., Zhu, F., Sonntag, A., Waldvogel, B., Vannier, C., Darling, D., zur Hausen, A., Brunton, V. G.,
Morton, J., Sansom, O., Schuler, J., Stemmler, M. P., Herzberger, C., Hopt, U., Keck, T., Brabletz, S., and Brabletz, T. (2009)
Nat Cell Biol 11, 1487-1495
Postsynaptic and differential localization to neuronal subtypes of protocadherin beta16 in the mammalian central nervous system.
Junghans, D., Heidenreich, M., Hack, I., Taylor, V., Frotscher, M. and Kemler, R. (2008)
Eur. J. Neurosci. 27, 559-571
Simultaneous loss of beta- and gamma-catenin does not perturb hematopoiesis or lymphopoiesis.
Koch, U., Wilson, A., Cobas, M., Kemler, R., Macdonald, H.R. and Radtke, F. (2008)
Blood 111, 160-164
A nonneural epithelial domain of embryonic cranial neural folds gives rise to ectomesenchyme.
Breau, M. A., Pietri, T., Stemmler, M. P., Thiery, J. P. and Weston, J. A. (2008)
Proc Natl Acad Sci U S A 105, 7750-7755
Cadherins in development and cancer.
Stemmler, M. P. (2008)
Mol Biosyst 4, 835-850
The Opitz syndrome gene product MID1 assembles a microtubule-associated ribonucleoprotein complex.
Aranda-Orgillés, B., Trockenbacher, A., Winter, J., Aigner, J., Köhler, A., Jastrzebska, E., Stahl, J., Müller, E.C., Otto, A., Wanker, E.E., Schneider, R., Schweiger, S. (2008)
Hum Genet. 123, 163-176
Comparative 3'UTR analysis allows identification of regulatory clusters that drive Eph/ephrin expression in cancer cell lines.
Winter J, Roepcke S, Krause S, Müller EC, Otto A, Vingron M, Schweiger S. (2008)
PLoS One 3, e2780
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